BMB 310 Methods in Biochemistry and Cell Biology


This course provides instruction in the theory and application of a variety of research techniques dealing with the structure and function
of proteins in biological systems. Students will gain practical laboratory experience in procedures including chromatographic separation
of proteins, spectrophotometric protein assays, kinetic characterization of enzymes, polyacrylamide gel electrophoresis, antibody
production, enzyme-linked immunosorbent assays (ELISA), immunoblotting, and fluorescence microscopy. Additional experience will
be gained in the practical application of computer-based bioinformatics tools for characterizing proteins based on their amino acid or gene sequences, as well as on mass-spectrometric analysis of peptide fragment fingerprints. This course can count as laboratory credit to accompany Biology 307 or Chemistry 414 or both. One hour of lecture and three hours of laboratory per week plus independent work.